The Anti-CD3 antibody is designed to react with the CD3 antigen expressed by the intracytoplasmic portion of T-cells. It can be used to stain human T-cells in the thymus medulla and cortex medulla, as well as peripheral lymphoid tissues. It is also suitable for the staining of neoplastic and normal T-cells using paraffin-embedded or Formalin-fixed tissues.
More Information
There is no clone for the Anti-CD3 antigen, and the immunogen is the synthetic 13-mer peptide which corresponds to the internal region of the CD3 protein and epsilon chain in humans. The IG isotype is the Rabbit IgG, and there is no determined epitope. Its molecular weight is 19kDa, and it has been tested in humans.
It is applied in Western Blotting and Immunohistochemistry applications. The IHC control is the tonsil and requires paraffin-embedded or Formalin-fixed tissues. The dilution ratio is 1:200 when using concentrated formats. To retrieve the antigen, you should boil your tissue sections using a 10mM citrate buffer with a pH of 6.0 for at least 10 minutes, cooling to room temperature for 20 minutes. Likewise, you’ll have an incubation period of 10 minutes.
For Western Blotting, it is recommended that you dilute the antibody with a ratio of 1:25 and incubate for one hour at room temperature. The positive control for Western Blotting is the Jurkat cell lysate with a cellular localization in the membrane.
Forms
You can purchase the Anti-CD3 antibody in 0.1 ml, 0.5 ml, and 1.0 ml immunogen affinity in a PBS/1 percent BSA buffer with a pH of 7.6. Likewise, it can also be found in a pre-diluted format of 7.0 ml with the same buffers and pH.
The Anti-CD3 antibody can be used to help stain various T-cells. Visit Spring Bioscience now for more information on how to purchase.
